KMID : 1094720150200010117
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Biotechnology and Bioprocess Engineering 2015 Volume.20 No. 1 p.117 ~ p.123
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Enhanced sulfate reduction by Citrobacter sp. coated with Fe3O4/SiO2 magnetic nanoparticles
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Zhou Wei
Yang Maohua Song Ziyu Xing Jianmin
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Abstract
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A sulfate-reducing Citrobacter strain was isolated and coated with Fe3O4/SiO2 magnetic nanoparticles (MNPs) to enhance sulfate reduction. Biolog analysis showed that it utilizes a broad range of electron donors. The findings also showed that this bacteria strain is a facultative anaerobe and can completely reduce 12 mM of sulfate to sulfide in 7 days under anaerobic conditions. Moreover, sulfate reduction was enhanced by 79% under optimized conditions. Different SiO2 wrap-ratios of the MNPs attached to the cell surface were studied to optimize the sulfate reduction: the surface of cells coated with 300% silica wrap-ratio MNPs showed the highest stability and increased desulfurization batch time, with a 450% increase in sulfate reduction in comparison with uncoated cells cultivated in anaerobic conditions.
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KEYWORD
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anaerobic processes, biodesulfurization, cell immobilization, Citrobacter sp. HCSR, magnetic particles (MNPs), waste-water treatment
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